María Amaya Aleixandre De Artiñano *

Autora invitada por SIIC

COMPUESTOS ANTIHIPERTENSIVOS DERIVADOS DE PROTEÍNAS DE HUEVO

En el presente estudio se evalúa el posible efecto antihipertensivo de los siguientes productos: la clara de huevo (CH), un hidrolizado de clara de huevo obtenido mediante tratamiento enzimático con pepsina (HCH), la fracción peptídica del HCH con masa molecular inferior a 3000 Da (HCH<3000 Da), y 3 secuencias peptídicas indentificadas en HCH<3000 Da (Tyr-Ala-Glu-Glu-Arg-Tyr-Pro-Ile-Leu = YAEERYPIL, Arg-Ala-Asp-His-Pro-Phe-Leu = RADHPFL, e Ile-Val-Phe = IVF). Los productos anteriormente mencionados se administraron por vía oral mediante sonda intragástrica a ratas espontáneamente hipertensas (SHR) y normotensas Wistar-Kyoto (WKY) de 17-20 semanas de vida. Se midió la presión arterial sistólica (PAS) y la presión arterial diastólica (PAD) en las ratas mediante el método del manguito en la cola, antes de la administración de los diferentes productos y también 2, 4, 6, 8 y 24 horas post-administración. HCH, HCH<3000Da y las tres secuencias peptídicas disminuyeron la PAS y la PAD en las ratas SHR pero no modificaron esas variables en las ratas WKY. La secuencias peptídicas YAEERYPIL, RADHPFL e IVF mostraron una potencia para disminuir la presión arterial mayor que HCH y HCH<3000 Da. Los resultados obtenidos sugieren que los productos estudiados podrían ser usados como ingredientes funcionales con potenciales efectos beneficiosos en la prevención y/o el tratamiento de la hipertensión arterial.

*María Amaya Aleixandre De Artiñano
describe para SIIC los aspectos relevantes de su trabajo
SHORT-TERM EFFECT OF EGG-WHITE HYDROLYSATE PRODUCTS ON THE ARTERIAL BLOOD PRESSURE OF HYPERTENSIVE RATS
Institución principal de la investigación
*Facultad de Medicina. Universidad Complutense de Madrid, Madrid, España
Imprimir nota

Referencias bibliográficas
Akpaffiong MJ, Taylor AA. (1998) Antihypertensive and vasodilator actions of antioxidants in spontaneously hypertensive rats. Am J Hypertens 11, 1450-1460.
Chen TL, Lo YC, Hu WT, Wu MC, Chen ST, Chang HM. (2003) Microencapsulation and modification of synthetic peptides of food proteins reduces the blood pressure of spontaneously hypertensive rats. J Agric Food Chem 51, 1671-1675.
Dávalos A, Miguel M, Bartolomé B, López-Fandiño R. (2004) Antioxidant activity of peptides derived from egg white proteins by enzymatic hydrolysis. Journal of Food Protection 67, 1939-1944.
Fujita H, Usui H, Kurahashi K, Yoshikawa M. (1995a) Isolation and characterization of ovokinin, a bradykinin B1 agonist peptide derived from ovalbumin. Peptides 16, 785-790.
Fujita H, Sasaki R, Yoshikawa M. (1995b) Potentiation of the antihypertensive activity of orally administered Ovokinin, a vasorelaxing peptide derived from ovalbumin, by emulsification in egg phosphatidyl-choline. Biosci Biotechnol Biochem 59, 2344-2345.
Fujita H, Yamagami T, Ohshima K. (2001) Effects of an ACE-inhibitory agent, katsuobushi oligopeptide, in the spontaneously hypertensive rat and in borderline and mildly hypertensive subjects. Nutr Res 21, 1149-1158.
Gobbetti M, Ferranti P, Smacchi E, Goffredi F, Addeo F. (2000) Production of angiotensin-I-converting-enzyme-inhibitory peptides in fermented milks started by Lactobacillus delbrueckii subsp. bulgaricus SS1 and Lactococcus lactis subsp. cremoris FT4. Appl Environ Microbiol 66, 3898-3904.
Karaki H, Doi K, Sugano S, Uchiwa H, Sugai R, Murakami U, Takemoto S. (1990) Antihypertensive effect of tryptic hydrolysate of milk casein in spontaneously hypertensive rats. Comp Biochem Physiol 96, 367–371.
Kawasaki T, Jun CJ, Fukushina Y, Kegai K, Seki E, Osajima K, Itoh K, Matsui T, Matsumoto K. (2002) Antihypertensive effect and safety evaluation of vegetable drink with peptides derived from sardine protein hydrolysated on mild hypertensive, high normal, and normal blood pressure subjects. Fukuoka Igaku Zasshi. 93, 208-218.
Matoba N, Usui H, Fujita H, Yoshikawa M. (1999) A novel antihypertensive peptide derived from ovalbumin induces nitric oxide-mediated vasorelaxation in an isolated SHR mesenteric artery. FEBS Letters 452, 181-184.
Matoba N, Yamada Y, Usui H, Nakagiri R, Yoshikawa M. (2001) Designing potent derivatives of Ovokinin(2-7), an anti-hypertensive peptide derived from ovalbumin. Biosci Biotechnol Biochem 65, 636-739.
Miguel M, Recio I, Gomez-Ruiz JA, Ramos M, Lopez-Fandiño R. (2004) Angiotensin I-converting enzyme inhibitory activity of peptides derived from egg white proteins by enzymatic hydrolysis. Journal of Food Protection 67, 1914-1920.
Miguel M, Muguerza B, Sánchez E, Delgado MA, Recio I, Ramos M, Aleixandre MA. (2005). Long-term blood pressure-lowering effect of milk fermented by enterococcus faecalis cect 5728 in spontaneously hypertensive rats. British Journal of Nutrition 93, 1-9
Mizushima S, Ohshige K, Watanabe J, Kimura M, Kadowaki T, Nakamura Y, Tochikubo O, Ueshima H. (2004) Randomized controlled trial of sour milk on blood pressure in borderline hypertensive men. Am J Hypertens 17, 701-706.
Nakamura Y, Masuda OY, Takano T. (1996) Decrease of tissue angiotensin I-converting enzyme activity upon feed sour milk in spontaneously hypertensive rats. Biosci Biotechnol Biochem 60, 488-489.
Nakamura Y, Yamamoto N, Sakai KY, Takano T. (1995) Antihypertensive effect of sour milk and peptides isolated from it that are inhibitors to angiotensin I converting enzyme. J Dairy Sci 78, 1253-1257.
Nimmaguda R. (2002) New bioactive peptides for the nutritional industry. Oral presentation at: SupplySide West Trade Show and Conference, Las Vegas, NV, 4-6 Diciembre.
Nurminen ML, Sipola M, Kaarto H, Pihlanto-Leppälä A, Piilola K, Korpela R, Tossavainen H, Korhonen H & Vapaatalo H (2000) a-Lactorphin lowers blood pressure measured by radiotelemetry in normotensive and spontaneously hypertensive rats. Life Sci 16, 1535–1543.
Okamoto K, Aoki K. (1963) Development of a strain of spontaneously hypertensive rats. Jpn Circ J 27, 282-293.
Rodríguez-Iturbe B, Zhan CD, Quiroz Y, Sindhu RF, Vaziri ND. (2003) Antioxidant-rich diet relieves hypertension and reduces renal immune infiltration in spontaneously hypertensive rats. Hypertension 41, 341.
Saito Y, Wanezaki K, Kawato A & Imayasu S (1994) Antihypertensive effects of peptide in sake and its by-products on spontaneously hypertensive rats. Biosci Biotechnol Biochem 58, 812–816.
Seppo L, Jauhiainen T, Poussa T, Korpela R. (2003) A fermented milk high in bioactive peptides has a blood pressure-lowering effect in hypertensive subjects. Am J Clin Nutr 77, 326-330.
Sipola M, Finckenberg P, Korpela R, Vapaatalo H, Nurminen ML. (2002) Effect of long-term intake of milk products on blood pressure in hypertensive rats. J Dairy Res 69, 103-111.
Soares de Moura R, Costa Viana FS, Souza MAV, Kovary K, Guedes DC, Oliveira EPB, Rubenich LSM, Carvalho LC, Oliveira RM, Tano RM, Gusmão T, Correia ML. (2002) Antihypertensive, vasodilator and antioxidant effects of a vinifiera grape skin extract. J Pharm Pharmacol 54, 515-1520.
Wu J, Ding X. (2001) Hypotensive and physiological effect of angiotensin converting enzyme inhibitory peptides derived from soy protein on spontaneously hypertensive rats. J Agric Food Chem 49, 501-506

Otros artículos de María Amaya Aleixandre De Artiñano

Miguel M, Muguerza B, Sánchez E, Delgado MA, Recio I, Ramos M, Aleixandre MA.
Changes in arterial blood pressure of milk fermented by Enterococcus faecalis CECT 5728 in spontaneously hypertensive rats.
British Journal of Nutrition (2005) 93, 1-9. (2,967, posición 9/53, Nutrition & Dietetics). 4 citaciones.

Miguel M, López-Fandiño R, Ramos M, Aleixandre MA.
Short-term effect of egg white hydrolysate products on the arterial blood pressure of hypertensive rats.
British Journal of Nutrition (2005) 94, 731-737. (2,967, posición 9/53, Nutrition & Dietetics). 15 citaciones.

Muguerza B, Ramos M, Sánchez E, Manso MA, Miguel M, Aleixandre A, Delgado MA, Recio I.
Antihypertensive activity of milks fermented by Enterococcus faecalis strains isolated from raw milk.
International Dairy Journal (2006) 16, 61-69. (2,519, posición 5/96, Food Science and Nutrition). 17 citaciones.

Miguel M, Ramos M, Aleixandre MA, López-Fandiño R.
Antihypertensive peptides obtained from egg white proteins by enzymatic hydrolysis. Stability under simulated gastrointestinal digestion.
Journal of Agricultural and Food Chemistry (2006) 54(3), 726-31. (2,322, posición 1/31, Agriculture Multidisciplinary). 8 citaciones.

Miguel M, López-Fandiño R, Ramos M, Aleixandre MA.
Long-term antihypertensive effect of egg white treated with pepsin in hypertensive rats.
Life Science (2006) 78, 2960-2966. (2,512, posición 28/79, Medicine, Research & Experimental). 7 citaciones.

Miguel M, Aleixandre MA.
Antihypertensive peptides from egg white proteins. Review.
Journal of Nutrition (2006) 136, 1457-1460. (4,009, posición 5/55, Nutrition & Dietetics). 5 citaciones.

M Miguel, I Recio, M Ramos, MA Delgado, MA Aleixandre.
Effect of ACE-Inhibitory Peptides Obtained from Enterococcus faecalis Fermented Milk in Hypertensive Rats.
Journal of Dairy Science (2006) 89(9), 3352-9. (2,284, posición 2/44, Agriculture, Dairy and Animal Science). 2 citaciones.

Ana Quirós, Mercedes Ramos, Begoña Muguerza, Marco A. Delgado, Marta Miguel, Amaya Aleixandre, Isidra Recio.
Identification of novel antihypertensive peptides in milk fermented with Enterococus faecalis.
International Dairy Journal (2007) 17, 33-41. (2,198, posición 13/103, Food Science and Nutrition). 2 citaciones.

Miguel M, Manso MA, Aleixandre MA, López-Fandiño R.
Angiotensin converting enzyme activity in plasma and tissues of spontaneously hypertensive rats after short- and long-term intake of an egg white hydrolysate.
Molecular Nutrition and Food Research (2007) 51, 555-563. (3,439, posición 3/103, Food Science and Nutrition). 2 citaciones.

Miguel M, Alonso MJ, Salaices M, Aleixandre MA, López-Fandiño R.
Antihypertensive, ACE-inhibitory and vasorelaxing properties of an egg white hydrolysate. Effect of a simulated intestinal digestion.
Food Chemistry (2007) 104, 163-168. (3,052, posición 4/103, Food Science and Nutrition). 3 citaciones.

Hernández-Ledesma B, Miguel M, Aleixandre MA, Recio I.
Effect of simulated gastrointestinal digestion on the antihypertensive properties of -lactoglobulin peptides.
Journal of Dairy Research (2007) 74 (3), 336-339. (1,507, posición 9/47, Agriculture, Dairy and Animal Science).
Miguel M, Manso M, Alonso MJ, López-Fandiño R, Aleixandre MA, Salaices M.
Vasodilator, ACE-inhibitory and antihypertensive activity of peptides obtained from egg white hydrolysates.
Journal Agricultural and Food Chemistry (2007) 55(26):10615-10621. (2,532, posición 1/35, Agriculture Multidisciplinary). 1 citaciones.

Manso MA, Miguel M, Even J, Hernandez R, Aleixandre MA, López-Fandiño R. Effect of the long-term intake of an egg white hydrolysate on the oxidative status and blood lipid profile of spontaneously hypertensive rats.
Food Chemistry (2008) 109: 361-367. (3,052, posición 4/103, Food Science and Nutrition).

Aleixandre MA, Miguel M, Begoña Muguerza.
Péptidos antihipertensivos derivados de leche y de huevo. Revisión.
Nutrición Hospitalaria (2008) 23(4): 313-318. Indexada desde el año 2006, factor de impacto en 2008.

Sánchez D, Muguerza B, Moulay L, Hernández, R, Miguel M, Aleixandre A.
Apple pectin fiber reduces cardiometabolic risk factors in Zucker fatty rats.
Journal of Agricultural ald Food Chemsitry (2008) 56: 3574-3581. (2,532, posición 1/35, Agriculture Multidisciplinary).

Marta Miguel, Mª del Mar Contreras, Isidra Recio, Amaya Aleixandre.
ACE-inhibitory and Antihypertensive Properties of a Bovine Casein Hydrolysate
Food Chemistry doi:10.1016/j.foodchem.2008.05.041. (3,052, posición 4/103, Food Science and Nutrition).

Para comunicarse con María Amaya Aleixandre De Artiñano mencionar a SIIC como referencia:
amaya@med.ucm.es

---

Acerca del trabajo completo

COMPUESTOS ANTIHIPERTENSIVOS DERIVADOS DE PROTEÍNAS DE HUEVO

Título original en castellano
COMPUESTOS ANTIHIPERTENSIVOS DERIVADOS DE PROTEINAS DE HUEVO

Autor
María Amaya Aleixandre De Artiñano¹, Marta Miguel²
1 Farmacólogo, Facultad de Medicina. Universidad Complutense de Madrid, Madrid, España, Profesor Titular de Universidad
2 Investigador, Facultad de Medicina. Universidad Autónoma de Madrid, Doctor Contratado

Acceso a la fuente original
British Journal of Nutrition
http://www.journals.cambridge.org/action/displayJournal?jid=BJN

El artículo se relaciona estrictamente con las especialidades de siicsalud
El artículo se conecta secundariamente con las especialidades