NUEVOS ARN INHIBIDORES DE VHC OBTENIDOS POR SELECCION IN VITRO





NUEVOS ARN INHIBIDORES DE VHC OBTENIDOS POR SELECCION IN VITRO

(especial para SIIC © Derechos reservados)
El método de selección molecular in vitro desarrollado constituye una potente herramienta terapéutica que permite la obtención de nuevos ARN antivirales.
Autor:
c Romero-lópez
Columnista Experto de SIIC

Institución:
Instituto de Parasitología y Biomedicina ´López-Neyra´


Artículos publicados por c Romero-lópez
Coautores
Cristina Romero López*  Alicia Barroso del Jesús**  Elena Puerta Fernández***  Alfredo Berzal Herranz**** 
Estudiante de Doctorado, Instituto de Parasitología y Biomedicina López Neyra, Estudiante*
Investigadora, Instituto de Parasitología y Biomedicina López Neyra, Consejo Superior de Investigaciones, Investigadora Contratada**
Investigadora, Universidad de Yale, Investigadora Posdoctoral***
Investigador, Instituto de Parasitología y Biomedicina López Neyra, Consejo Superior de Investigaciones, Investigador Científico****
Aprobación
22 de Julio, 2005
Primera edición
28 de Abril, 2006
Segunda edición, ampliada y corregida
7 de Junio, 2021

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Extensión:  +/-2.67 páginas impresas en papel A4
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Clasificación en siicsalud
Artículos originales > Expertos de Iberoamérica >
página   www.siicsalud.com/des/expertocompleto.php/

Especialidades
Principal: Bioquímica
Relacionadas: Diagnóstico por Laboratorio, Gastroenterología, Infectología, Medicina Interna



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Bibliografía del artículo
Aldaz Carroll L, Tallet B, Dausse E, Yurchenko L, Toulmé JJ. (2002). Apical loop-internal loop interactions: a new RNA-RNA recognition motif identified through in vitro selection against RNA hairpins of the hepatitis C virus mRNA. Biochemistry 41, 5883-5893.
Barroso del Jesús A, Tabler M, Berzal Herranz A. (1999). Comparative kinetic analysis of structural variants of the hairpin ribozyme reveals further potential to optimize its catalytic performance. Antisense Nucleic Acid Drug Dev 9:433-440.
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Honda M, Beard MR, Ping LH, Lemon SM. (1999). A phylogenetically conserved stem-loop structure at the 5' border of the internal ribosome entry site of hepatitis C virus is required for cap-independent viral translation. J Virol 73:1165-1174.
Honda M, Ping LH, Rijnbrand RC, Amphlett E, Clarke B, Rowlands D, Lemon SM. (1996). Structural requirements for initiation of translation by internal ribosome entry within genome-length hepatitis C virus RNA. Virology 222:31-42.
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Kikuchi K, Umehara T, Fukuda K, Hwang J, Kuno A, Hasegawa T, Nishikawa S. (2003). RNA aptamers targeted to domain II of hepatitis C virus IRES that bind to its apical loop region. J Biochem (Tokyo) 133:263-270.
Korf M, Jarczak D, Beger C, Manns MP, Kruger M. (2005). Inhibition of hepatitis C virus translation and subgenomic replication by siRNAs directed against highly conserved HCV sequence and cellular HCV cofactors. J Hepatol 43:225-234.
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Pérez Ruiz M, Torres C, García López PA, Ruiz Extremera A, Salmerón J, Berzal Herranz A. (1997). Determination of HCV RNA concentration by direct quantitation of the products from a single RT-PCR. J Virol Methods 69:113-124.
Prabhu R, Vittal P, Yin Q, Flemington E, Garry R, Robichaux WH, Dash S. (2005). Small interfering RNA effectively inhibits protein expression and negative strand RNA synthesis from a full-length hepatitis C virus clone. J Med Virol 76:511-519.
Puerta Fernández E, Barroso del Jesús A, Romero López C, Berzal Herranz A. (2003a). HIV-1 TAR as anchoring site for optimized catalytic RNAs. Biol Chem 384:343-350.
Puerta Fernández E, Romero López C, Barroso del Jesús A, Berzal Herranz A. (2003b). Ribozymes: recent advances in the development of RNA tools. FEMS Microbiol Rev 27:75-97.
Reynolds JE, Kaminski A, Kettinen HJ, Grace K, Clarke BE, Carroll AR, Rowlands DJ, Jackson RJ. (1995). Unique features of internal initiation of hepatitis C virus RNA translation. Embo J 14:6010-6020.
Soler M, McHutchison JG, Kwoh TJ, Dorr FA, Pawlotsky JM. (2004). Virological effects of ISIS 14803, an antisense oligonucleotide inhibitor of hepatitis C virus (HCV) internal ribosome entry site (IRES), on HCV IRES in chronic hepatitis C patients and examination of the potential role of primary and secondary HCV resistance in the outcome of treatment. Antivir Ther 9:953-968.
Tallet López B, Aldaz Carroll L, Chabas S, Dausse E, Staedel C, Toulmé JJ. (2003). Antisense oligonucleotides targeted to the domain IIId of the hepatitis C virus IRES compete with 40S ribosomal subunit binding and prevent in vitro translation. Nucleic Acids Res 31:734-742.
Theissen G, Richter A, Lukacs N. (1989). Degree of biotinylation in nucleic acids estimated by a gel retardation assay. Anal Biochem 179:98-105.
Wakita T, Moradpour D, Tokushihge K, Wands JR. (1999). Antiviral effects of antisense RNA on hepatitis C virus RNA translation and expression. J Med Virol 57:217-222.
Wang TH, Rijnbrand RC, Lemon SM. (2000). Core protein-coding sequence, but not core protein, modulates the efficiency of cap-independent translation directed by the internal ribosome entry site of hepatitis C virus. J Virol 74:11347-11358.

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