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A AMEBIASE DEVERIA DETECTAR-SE COM METODOLOGIAS DIAGNOSTICAS MAIS ECONOMICAS E ESPECIFICAS
(especial para SIIC © Derechos reservados)
A (La) ameb√≠ase √© uma (es una) infec√ß√£o parasit√°ria causada pela (por) Entamoeba histolytica, protozo√°rio que representa um risco √† sa√ļde nos (un riesgo para la salud en los) pa√≠ses onde as (donde las) barreiras sanit√°rias s√£o inadequadas.
Autor:
Fred Luciano Neves Santos
Columnista Experto de SIIC

Institución:
Centro de Pesquisas Aggeu Magalh√£es


Artículos publicados por Fred Luciano Neves Santos
Recepción del artículo
28 de Septiembre, 2014
Aprobación
5 de Noviembre, 2014
Primera edición
9 de Diciembre, 2014
Segunda edición, ampliada y corregida
27 de Junio, 2017

Resumen
A (La) ameb√≠ase √© uma (es una) infec√ß√£o parasit√°ria causada pela (por) Entamoeba histolytica, protozo√°rio que representa um risco √† sa√ļde nos (un riesgo para la salud en los) pa√≠ses onde as (donde las) barreiras sanit√°rias s√£o inadequadas. Estima-se que 500 milh√Ķes de indiv√≠duos em todo o mundo estejam (se encuentran) infectados por este pat√≥geno, havendo at√© (hay hasta) 100 000 √≥bitos anuais (muertes anuales). O (El) diagn√≥stico laboratorial √© realizado rotineiramente atrav√©s da (rutinariamente a trav√©s de la) demonstra√ß√£o microsc√≥pica de cistos e/ou (o) trofozo√≠tos no (en el) sedimento fecal, n√£o permitindo a (no permitiendo la) detec√ß√£o de varia√ß√Ķes interespec√≠ficas entre a E. histolytica e esp√©cies n√£o patog√™nicas como a Entamoeba dispar e a (y) Entamoeba moshkovskii . Metodologias imunol√≥gicas para a pesquisa de anticorpos contra a (la b√ļsqueda de anticuerpos contra) E. histolytica j√° foram usadas e s√£o (ya fueron utilizadas y son) capazes de diferenciar as esp√©cies do (entre las especies del) complexo E. histolytica/dispar/moshkovskii, apesar de apresentarem resultados falso positivos e negativos. De modo semelhante a pesquisa de ant√≠genos espec√≠ficos por t√©cnicas imunol√≥gicas s√£o de utilidade limitada. A rea√ß√£o em cadeia da (La reacci√≥n en cadena de la) polimerase ainda constitui a metodologia de escolha para o (todav√≠a es la metodolog√≠a de elecci√≥n para el) diagn√≥stico espec√≠fico da ameb√≠ase, entretanto metodologias mais recentes foram empregadas no (fueron empleadas en el) diagn√≥stico da ameb√≠ase levando a resultados promissores (promisorios). A PCR em tempo real e as tecnologias baseadas na (basadas en la) detec√ß√£o de microesferas magn√©ticas acopladas foram utilizadas para o diagn√≥stico da ameb√≠ase e apresentaram bom desempenho. Apesar do avan√ßo no (del avance en el) diagn√≥stico laboratorial da ameb√≠ase ao longo dos anos, ainda h√° necessidade do desenvolvimento de (a lo largo de los a√Īos, todav√≠a hay necesidad de desarollar) uma metodologia barata e espec√≠fica para a E. histolytica que possa ser utilizada nos laborat√≥rios cl√≠nicos em todo o mundo em substitui√ß√£o √†s (de las) t√©cnicas ultrapassadas utilizadas at√© hoje (hasta hoy).

Palabras clave
amebíase, diagnóstico, Entamoeba histolytica


Artículo completo

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Extensión:  +/-12.84 páginas impresas en papel A4
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Abstract
Amebiasis is a parasitic infection caused by protozoa Entamoeba histolytica and represents a serious health problem in areas where hygiene conditions and treatment of water supply are unsatisfactory. An estimate suggests that approximately 500 million people are infected globally each year and that up to 100 000 deaths per year can be attributed to complications caused by amebiasis. The laboratory diagnosis of amebiasis is performed by traditional light microscopy by demonstrating cysts and/or trophozoites in faeces. However, its ability to detect interspecies variation within E. histolytica, Entamoeba dispar and Entamoeba moshkovskii species is limited, making a presumptive diagnosis impossible. Immunological methods used to search for anti-E. histolytica antibodies are able to distinguish the species E. histolytica, E. dispar and E. moshkovskii species, although false-positive and false-negative results could be provided. Similarly, the search for specific antigens by immunologic tools is limited. Chain reaction polymerase is the method of choice for amebiasis diagnosis, however new methodologies were employed to make an amebiasis diagnosis and have been giving promising results. Similarly, real time PCR and magnetic beads-based technologies were used for amebiasis diagnosis and have demonstrated an excellent performance. Despite the advances in laboratory amebiasis diagnosis over the years, it is necessary to develop cheaper methodologies that are specific to the E. histolytica and which can be used in clinical laboratories worldwide to replace the obsolete methodologies used currently.

Key words
amebiasis, diagnosis, Entamoeba histolytica


Clasificación en siicsalud
Artículos originales > Expertos de Iberoamérica >
página   www.siicsalud.com/des/expertocompleto.php/

Especialidades
Principal: Diagn√≥stico por LaboratorioInfectolog√≠a
  Relacionadas: Epidemiolog√≠aSalud P√ļblicaBioqu√≠mica

Enviar correspondencia a:
Fred Luciano Neves Santos, 50.670-420, Av. Professor Moraes Rego, s/n - Campus da UFPE - Cidade Universit√°ria, Recife, Brasil
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