Marco T. Rincón *

Autor invitado por SIIC

El trabajo revela un arreglo inusual de enzimas en el celulosoma de bacterias anaeróbicas celulolíticas

UNA PROTEINA ADAPTADORA QUE AUMENTA EL REPERTORIO DE ENZIMAS EN EL CELULOSOMA DE RUMINOCOCCUS FLAVEFACIENS

Se identificó un nuevo gen –denominado scaC– que codifica para una nueva proteína estructural del celulosoma. Este gen interactúa específicamente con otra proteína estructural del celulosoma, ScaA, y a su vez es capaz de interactuar con proteínas nativas con dominios de anclaje (dockerin) aún no identificados. Los datos presentados sugieren que el papel de ScaC es el de una proteína adaptadora por medio de la cual otros polipéptidos se ensamblan en el celulosoma.

*Marco T. Rincón
describe para SIIC los aspectos relevantes de su trabajo
SCAC, AN ADAPTOR PROTEIN CARRYING A NOVEL COHESIN THAT EXPANDS THE DOCKERIN-BINDING REPERTOIRE OF THE RUMINOCOCCUS FLAVEFACIENS 17 CELLULOSOME
Institución principal de la investigación
*Rowett Research Institute, Microbial Genetics Group, Gut Microbiology and Immunology Division, Aberdeen, Reino Unido, Aberdeen, Reino Unido
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Referencias bibliográficas
Aurilia VJ, Martin C, McCrae SI, Scott KP, Rincón MT, Flint HJ. 2000. Three multidomain esterases from the cellulolytic rumen anaerobe Ruminococcus flavefaciens 17 that carry divergent dockerin sequences. Microbiology 146 (Pt 6):1391-1397.Bayer EA, Chanzy H, Lamed R, Shoham Y. 1998a. Cellulose, cellulases and cellulosomes. Curr. Opin. Struct. Biol. 8:548-557.Bayer EA and Lamed R. 1986. Ultrastructure of the cell surface cellulosome of Clostridium thermocellum and its interaction with cellulose. J. Bacteriol. 167:828-836.Bayer EA, Shimon LJ, Shoham Y, Lamed R. 1998b. Cellulosomes-structure and ultrastructure. J. Struct. Biol. 124:221-234.Beguin P and Lemaire M. 1996. The cellulosome: an exocellular, multiprotein complex specialized in cellulose degradation. Crit Rev. Biochem. Mol. Biol. 31:201-236.Cunningham C, McPherson CA, Martin J, Harris WJ, Flint HJ. 1991. Sequence of a cellulase gene from the rumen anaerobe Ruminococcus flavefaciens 17. Mol. Gen. Genet. 228:320-323.Ding SY, Rincón MT, Lamed R, Martin JC, McCrae SI, Aurilia V, Shoham Y, Bayer EA, Flint HJ. 2001. Cellulosomal scaffoldin-like proteins from Ruminococcus flavefaciens. J. Bacteriol. 183:1945-1953.Julliand V, De Vaux A, Millet L and Fonty G. 1999. Identification of Ruminococcus flavefaciens as the predominant cellulolytic bacterial species of the equine cecum. Appl. Environ. Microbiol. 65:3738-3741.Kopecny J, Hajer J, Mrazek J. 2004. Detection of cellulolytic bacteria from the human colon. Folia Microbiol. (Praha) 49:175-177.Rincón MT, Ding SY, McCrae SI, Martin JC, Aurilia V, Lamed R, Shoham Y, Bayer EA, FlintHJ. 2003. Novel organization and divergent dockerin specificities in the cellulosome system of Ruminococcus flavefaciens. J. Bacteriol. 185:703-713.Rincón MT, McCrae SI, Kirby J, Scott KP, Flint HJ. 2001. EndB, a multidomain family 44 cellulase from Ruminococcus flavefaciens 17, binds to cellulose via a novel cellulose-binding module and to another R. flavefaciens protein via a dockerin domain. Appl. Environ. Microbiol. 67:4426-4431.Robert C and Bernalier-Donadille A. 2003. The cellulolytic microflora of the human colon: evidence of microcrystalline cellulose-degrading bacteria in methane-excreting subjects. FEMS Microbiol. Ecol. 46:81-89.Varel VH, Fryda SJ, Robinson IM. 1984. Cellulolytic bacteria from pig large intestine. Appl. Environ. Microbiol. 47:219-221.

Otros artículos de Marco T. Rincón

Domínguez-Bello MG, Lovera M, Rincón MT. (1997). Characteristics of dihydroxypyridine-degrading activity in the rumen bacteria Synergistes jonesii. FEMS Microbiol. Ecology. 23:361-365.Rincón MT, Allison MJ, Michelangeli F, De Sanctis Y, Domínguez-Bello MG. (1998). Anaerobic degradation of mimosine-derived hydroxypyridines by cell free extracts of the rumen bacterium Synergistes jonesii. FEMS Microbiol Ecology. 27:127-132Flint HJ, Aurilia V, Kirby J, Miyazaki K, Rincón MT, McCrae S, Martin J. (1999). Organization of plant cell wall degrading enzymes in the anaerobic bacteria Ruminococcus flavefaciens and Prevotella bryantii. in Genetics, Biochemistry and Ecology of Cellulose Degradation. (K Ohmiya, K Hayashi, K Sakka, Y Kobayashi, S Karita and T Kimura, eds.) Proceedings of MIE BIOFORUM 98: Genetics, Biochemistry and Ecology of Cellulose Degradation. UNI Publisher Co. Ltd. Tokio .pp 511-520.Aurilia V, Martin JC, McCrae SI, Scott KP, Rincón MT, Flint HJ. (2000) Three multidomain esterases from the cellulolytic rumen anaerobe Ruminococcus flavefaciens 17 that carry divergent dockerin sequences. Microbiology 146:1391-1397.Rincón MT, Domínguez-Bello MG, Lovera M and Romero MR (2000). Degradation of toxic pyridinediols derived from mimosine by ruminal bacteria: I. Microbiological aspects. Revista Científica de la Faculta de Veterinaria-LUZ 10:311-318.Ding SY, Rincón MT, Lamed R, Martin J, McCrae SI, Aurilia V, Shoham Y, Bayer E and Flint HJ (2001). Cellulosome scaffoldin-like proteins from Ruminococcus flavefaciens. J. Bacteriol. 183(6):1945-1953.Rincón MT, McCrae SI, Kirby J, Scott KP and Flint HJ (2001). EndB, a newly identified family 44 cellulase from the rumen cellulolytic bacterium Ruminococcus flavefaciens 17, binds to cellulose via a novel cellulose-binding module and to another R. flavefaciens protein via a dockerin domain. Applied and Environmental Microbiology 67(10):4426-4431.Rincón MT, Ding SY, McCrae SI, Martin JC, Aurilia V, Lamed R, Shoham Y, Bayer E, Flint HJ (2003) Evidence for novel cellulosome organization and for the formation of more than one type of plant cell wall degrading enzyme complex in the rumen cellulolytic bacterium Ruminococcus flavefaciens. J. Bacteriol. 185(3):703-713.

Para comunicarse con Marco T. Rincón mencionar a SIIC como referencia:
mtr@rowett.ac.uk

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Acerca del trabajo completo

UNA PROTEINA ADAPTADORA QUE AUMENTA EL REPERTORIO DE ENZIMAS EN EL CELULOSOMA DE RUMINOCOCCUS FLAVEFACIENS

Título original en castellano
SCAC, UNA PROTEINA ADAPTADORA CON UN NUEVO DOMINIO DE COHESION QUE EXPANDE EL REPERTORIO DE INTERACCION CON PROTEINAS POSEEDORAS DE DOMINIOS DE ANCLAJE EN EL CELULOSOMA DE RUMINOCOCCUS FLAVEFACIENS 17

Autor
Marco T. Rincón¹, Jennifer C. Martin², Vincenzo Aurilia³, Sheila I. McCrae⁴, Garry J. Rucklidge⁵, Martin D. Reid⁶, Edward A. Bayer⁷, Raphael Lamed⁸, Harry J. Flint⁹
1 Médico Veterinario, Rowett Research Institute, Investigador
2 Técnico, Rowett Research Institute, Técnico de Laboratorio
3 Biologo, Istituto di Biochimica Delle Proteine, Consiglio Nazionale delle Ricerche, Nápoles, Italia, Investigador
4 Técnico, Rowett Research Institute, Técnico
5 Biologist, Rowett Research Institute, Director of Proteomic Unit
6 Técnico, Rowett Research Institute, Técnico
7 Structural Biologist, Weizman Institute, Israel, Investigador
8 Biologist, Tel Aviv University, Professor
9 Microbial Geneticist, Rowett Research Institute, Head of Gut Microbiology & Immunology Division

Acceso a la fuente original
Journal of Bacteriology
http://jb.asm.org/