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Maria Isabel Nogueira Cano *

Autora invitada por SIIC


PROTEINAS TELOMERICAS EM PROTOZOARIOS DO GENERO LEISHMANIA

Neste trabalho mostramos a identificaçao e caracterizaçao de tres proteinas telomericas de Leishmania amazonensis alem da presença de atividade de telomerase em extratos do parasita. Para isso utilizou-se ensaios cromatográficos, ensaios de interação proteína-DNA, testes de extensao de sequencias telomericas e espectrometria de massa.

*Maria Isabel Nogueira Cano
describe para SIIC los aspectos relevantes de su trabajo
IDENTIFICATION OF THREE PROTEINS THAT ASSOCIATE IN VITRO WITH THE LEISHMANIA (LEISHMANIA) AMAZONENSIS G-RICH TELOMERIC STRAND
European Journal of Biochemistry,
271(14):3050-3063 Jul, 2004

Esta revista, clasificada por SIIC Data Bases, integra el acervo bibliográfico
de la Biblioteca Biomédica (BB) SIIC.

Institución principal de la investigación
*Departamento de Patología Clínica Facultad de Ciencias Médicas Universidad Estatal de Campinas (UNICAMP) SP, Brasil, Campinas, Brasil
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Referencias bibliográficas
1. Fu G & Barker D. (1998) Characterisation of Leishmania telomeres reveals unusual telomeric repeats and conserved telomere-associated sequence. Nucleic Acids Res. 26, 2161-2167.
2. Henderson E. (1995) Telomere DNA Structure. In: Telomeres, Blackburn, E. & Greider, C.- Cold Spring Harbor Laboratory Press, pp. 11-34.
3. Henderson E. & Blackburn, E.H. (1989) An overhanging 3’ terminus is a conserved feature of telomeres. Mol. Cell. Biol. 9, 345-348.
4. Greider, C. (1996) Telomere Length regulation. Annu. Rev. Biochem. 65, 337-365.
5. Chan, S.W-L. & Blackburn, E.H. (2002) New ways not to make ends meet: telomerase, DNA damage proteins and heterochromatin. Oncogene 21, 553-563.
6. McElligott, R. & Wellinger, R.J. (1997) The terminal DNA structure of mammalian chromosomes. EMBO J. 16, 3705-3714.
7. Wellinger, R.J., Wolf, A.J., Zakian, V. (1993) Saccharomyces telomeres acquire single-strand TG1-3 tails late in s phase. Cell 72, 51-60.
8. Wellinger, R.J., Ethier, K., Labrecque, P., Zakian, V.A. (1996) Evidence for a new step in telomere maintenance. Cell 85, 423-433.
9. de Lange, T. (2002) Protection of mammalian telomeres. Oncogene 21, 532-540.
10. Blackburn, E.H. (2000) Telomere states and cell fates. Nature 408, 53-56.
11. Blackburn, E.H. (2001) Switching and signaling at the telomere. Cell 106, 661-673.
12. Lin, J.J. & Zakian, V.A. (1996) The Saccharomyces CDC13 protein is a single-strand TG1-3 telomeric DNA-binding protein in vitro that affects telomere behavior in vivo. Proc. Natl. Acad. Sci. USA 93, 13760-13765.
13. Nugent, C., Hughes, T., Lue, N., Lundblad, V.J. (1996) Cdc13p: A single-strand telomeric DNA-binding protein with a dual role in yeast telomere maintenance. Science 274, 249-252.
14. Chandra, A., Hughes, T.R., Nugent, C.I., Lundblad, V. (2001) Cdc13 both positively and negatively regulates telomere replication. Genes Dev. 15, 404-414.
15. Evans, S.K. & Lundblad, V.J. (1999) Est1 and Cdc13 as comediators of telomerase access. Science 286, 117-120.
16. Evans S.K. & Lundblad V.J. (2000) Positive and negative regulation of telomerase access to the telomere. J. Cell Sci. 19, 3357-3364.
17. Grandin, N., Reed, S.I., Charbonneau, M. (1997) Stn1, a new Saccharomyces cerevisiae protein, is implicated in telomere size regulation in association with Cdc13. Genes Dev. 11, 512-527.
18. Grandin, N., Damon, C. & Charbonneau, M. (2001) Ten1 functions in telomere end protection and length regulation in association with Stn1 and Cdc13. EMBO J. 20,1173-83.
19. Qi, H. & Zakian, V.A. (2000) The Saccharomyces telomere-binding protein Cdc13p interacts with both the catalytic subunit of DNA polymerase a and the telomerase-associated Est1 protein. Genes Dev. 14, 1777-1788.
20. Baumann, P., Cech, T.R. (2001) Pot1, the putative telomere end-binding protein in fission yeast and human. Science 292, 1171-1175.
21. Colgin, L.M., Baran, K., Baumann, P, Cech, T.R. & Reddel, R.R. (2003) Human POT1 facilitates telomere elongation by telomerase. Curr. Biol. 13, 942–946.
22. Loayza, D. & de Lange, T. (2003) POT1 as a terminal transducer of TRF1 telomere length control. Nature. 26, 1013-1018.
23. Cano, M.I.N., Dungan, J., Agabian, N., Blackburn, E.H. (1999) Telomerase in kinetoplastid parasitic protozoa. Proc. Natl. Acad. Sci. USA 96, 3616-3621.
24. Cano, M.I.N. (2001) Telomere biology of trypanosomatids: more questions than answers. Trends in Parasitology 17, 425-429.
25. Barry, J.D., Ginger, M.L., Burton, P., McCulloch, R. (2003) Why are parasite contingency genes often associated with telomeres? Int. J. Parasitol. 33, 29-45.
26. Eid, J. & Sollner-Webb, B. (1995) ST-1 a 39-kilodalton protein in Trypanosoma brucei, exhibist a dual affinity for the duplex form of the 29-base-pair subtelomeric repeat and its C-rich strand. Mol. Cell. Biol. 15, 389-397.
27. Eid, J. & Sollner-Webb, B. (1997) ST-2, a Telomere and subtelomere duplex and G-strand binding protein activity in Trypanosoma brucei. J. Biol. Chem. 272, 14927-14936.
28. Cano, M.I.N., Blake, J.J., Blackburn, E.H., Agabian, N. (2002) A Trypanosoma brucei protein complex that binds G-overhangs and co-purifies with telomerase activity. J. Biol. Chem. 277, 896-906.
29. Bryan, T.M. & Cech, T.R. (1999) Telomerase and the maintenance of chromosome ends. Curr. Opin. Cell Biol. 11, 318-324.
30. Field, H. & Field, M. (1996) Leptomonas seymouri, Trypanosoma brucei: a method for isolating trypanosomatid nuclear factors which bind T. brucei ss G-rich telomere sequence. Exp. Parasitol. 83, 155-158.
31. Sbicego, S., Alfonzo, J.D., Estevez, A.M., Rubio, M.A., Kang, X., Turck, C.W., Peris, M., Simpson, L. (2003) RBP38, a novel RNA-binding protein from trypanosomatid mitochondria, modulates RNA stability. Eukaryot. Cell. 2, 560-568.
32. Noll, T.M., Desponds, C., Belli, S.I., Glaser, T.A., Fasel, N.J. (1997) Histone H1 expression varies during the Leishmania major life cycle. Mol. Biochem. Parasitol. 84, 215–227.
33. Sambrook, J. & Russel, D.W. (2001) Molecular Cloning: a Laboratory Manual. 3rd Ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
34. Schnapp, G., Rodi, H.-P., Rettig, W.J., Schnapp, A., Damm, K. (1998) One-step affinity purification protocol for human telomerase. Nucleic Acids Res. 26, 3311-3313.
35. Kim, N., Piatyszek, M., Prowse, K., Harley, C., West, M., Ho, P., Coviello, G., Wright, W., Weinrich, S., Shay, J. (1994) Specific association of human telomerase activity with immortal cell and cancer. Science 266, 2011-2015.
36. Duhagon, M.A., Dallagiovanna, B., Ciganda, M., Ruyechan, W., Williams, N. & Garat, B. (2003) A novel type of single-stranded nucleic acid binding protein recognizing a highly frequent motif in the intergenic regions of Trypanosoma cruzi. Biochem. Biophys. Res. Commun. 309, 183-188.
37. Wold, M.S. (1997) Replication Protein A: a heterotrimeric, ssDNA-binding protein required for eukaryotic DNA metabolism. Annu. Rev. Biochem. 66, 61–91.
38. Smith, J., Zou, H., Rothstein, R. (2000) Characterization of genetic interactions with RFA1: the role of RPA in DNA replication and telomere maintenance. Biochimie 82, 71-78.
39. Chiurillo, M.A., Cano, M.I., Franco, J.S., Ramirez, J.L. (1999) Organization of telomeric and sub-telomeric regions of chromosome from the protozoan parasite Trypanosoma cruzi. Mol. Biochem. Parasitol. 100, 173-183.
40. Price, C.M., Cech, T.R. (1987) Telomeric DNA-protein interactions of Oxyticha macronuclear DNA. Genes Dev. 1, 783-793.
41. Virta-Pearlman, V., Morris, D.K. & Lundblad V. (1996) Est1 has the properties of a single-stranded telomere end-binding protein. Genes Dev. 10, 3094-104.
42. Johnston, S., Lew, J., Berman, J. (1999) Gbp1, a protein with RNA recognition motifs, binds ss telomeric DNA and changes its binding specificity upon dimerization. Mol. Cell. Biol. 19, 923-933.
43. Privalov, P.L. & Gill, S.J. (1988) Stability of protein structure and hydrophobic interaction. Adv. Protein Chem. 39, 191-234.
44. Bessler, J.B., Torredagger, J.Z., Zakian, V.A. (2001) The Pif1p subfamily of helicases: region-specific DNA helicases? Trends Cell. Biol. 11, 60-65.
45. Bochkareva, E., Korolev, S., Less-Miller, S.P. & Bochkarev, A. (2002) Structure of the RPA trimerization core and its role in the multistep DNA-binding mechanism of RPA. EMBO J. 21, 1855-1863.
46. Murzin, A.G. (1993) OB (oligonucleotide/oligosaccharide binding)- fold: common structural and functional solution for non-homologous sequences. EMBO J.12, 861-867.
47. Bochkareva, A., Pfuetzner, R.A., Edwards, A.M. & Frappier, L. (1997) Structure of the single-stranded-DNA-binding domain of replication protein A bound to DNA. Nature 385, 176-181.
48. Wang, W., Skopp, R., Scofield, M. & Price, C. (1992) Euplotes crassus has genes encoding telomere-binding proteins and telomere-binding protein homologs. Nucleic Acids Res. 20, 6621-6629.
49. Mitton-Fry R.M., Anderson, E.M., Hughes, T.R., Lundblad, V. & Wuttke, D.S. (2002) Conserved structure for single-stranded telomeric DNA recognition. Science 296, 145-147.
50. Schramke, V., Luciano, P., Brevet, P., Guillot, S., Corda, Y., Longhese, M.P., Gilson, E & Géli, V. (2004) RPA regulates telomerase action by providing Est1p access to chromosome ends. Nature Gen. 36, 46-54.
51. Opresko, P.L., von Kobbe, C., Laine, J.-P., Harrigan, J., Hickson, I.D. & Bohr, V. (2002) Telomere-binding protein TRF2 binds to and stimulates the Werner and Bloom Syndrome helicases. J. Biol. Chem. 25, 41110-41119.
52. Wong D, Pavlovich JG & Reich NO. (1998) Electrospray ionization mass spectrometric characterization of photocrosslinked DNA-EcoRI DNA methyltransferase complexes. Nucleic Acids Res. 26, 645-649.
Otros artículos de Maria Isabel Nogueira Cano

1. Fernández MF, Castellari RR, Conte FF, Gozzo F, Sabino AA, Pinheiro H, Novello JC, Eberlin MN, Cano MIN. (2004) Identification of three proteins that associate in vitro with the Leishmania (Leishmania) amazonensis G-rich telomeric strand. European Journal of Biochemistry, 271, 3050-3063.

2. Cano MI, Blake JJ, Blackburn EH, Agabian N. (2002) A Trypanosoma brucei protein that binds G overhangs and co-purifies with telomerase activity. Journal of Biological Chemistry, 277, 896-906.

3. Cano MI. Telomere Biology of Kinetoplastida protozoa: The little we know (Opinion) (2001) Trends in Parasitology, 17, 9425-9429.

4. Chiurillo M, Cano MI, Franco da Silveira J, Ramirez JL. (1999) Structure and organization of telomeric and subtelomeric regions of chromosomes from the protozoan parasite Trypanosoma cruzi. Molecular and Biochemical Parasitology, 100, 173-183.

5. Cano MI, Dungan J, Agabian N, Blackburn EH. (1999) Telomerase in kinetoplastid parasitic protozoa. Proceedings of the National Academy of Sciences USA, 96, 3616-3621.

6. Cano MI, Cisalpino P, Galindo I, Ramirez JL, Mortara R, Franco da Silveira J. (1998) Separation of Paracoccidioides brasiliensis chromosome-sized DNA molecules by pulsed field gel electrophoresis. Journal of Clinical Microbiology, 36, 742-747.

7. Zingales B, Pereira ME, Oliveira RP, Almeida KA, Umezawa ES, Souto RP, Vargas N, Cano MI, Da Silveira JF, Nehme NS, Morel CM, Brener Z, Macedo A. (1997) Trypanosoma cruzi genome project: biological characteristics and molecular typing of clone CL Brener. Acta Tropica, 29, 1-15.

8. Araya J, Cano MI, Novak EM, Gomes HBM, Guevara P, Ramires JL, Franco da Silveira J. (1997) A novel reiterated family of interspersed DNA elements in the genome of Trypanosoma cruzi. Parasitology, 115, 563-570.

9. Cisalpino P, Puccia R, Yamauchi L, Cano MI, Franco da Silveira J, Travassos LR. (1995) Cloning, characterization and epitope expression of the major diagnostic antigen of Paracoccidioides brasiliensis. Journal of Biological Chemistry, 271, 4553-4560.

10. Cano MI, Gruber A, Vasquez M, Cortes A, Levin MJ, Gonzalez A, Degrave W, Rondinelli E, Zingales E, Ramirez Jl, Alonso C, Requema JM, Franco da Silveira J. (1995) Trypanosoma cruzi genome project: Molecular karyotype of clone CL Brener. Molecular and Biochemical Parasitology, 71, 273-278.


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Autora invitada
27 de agosto, 2004
Descripción aprobada
15 de noviembre, 2004
Reedición siicsalud
3 de junio, 2010

Acerca del trabajo completo
PROTEINAS TELOMERICAS EM PROTOZOARIOS DO GENERO LEISHMANIA

Título original en castellano
Identificação de tres proteinas que se associam in vitro com a fita telomerica rica em G de Leishmania (Leishmania) amazonensis

Autor


Acceso a la fuente original
European Journal of Biochemistry
http://www.ejb.org/

El artículo se relaciona estrictamente con las especialidades de siicsalud


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