Conceptos Categóricos

Authors' Reports

Rahul Halkai
describes for SIIC the most significant aspects of his article
describe para SIIC los aspectos relevantes de su artículo


Aim: To address the cause of persistent infection of root canal treated teeth. Materials and Methods: 60 human single-rooted teeth divided into three groups. Group I (control group): no access opening and one-third of the apical root sealed using varnish. Group II: no preparation of teeth samples. In group III, apical root cementum was exposed to lactic acid and roughened using diamond point to mimic apical resorption. In group II & III E. faecalis broth was inoculated in the root canals and apical one-third of the tooth was immersed in the broth for 8 weeks with alternate day refreshment followed by biomechanical preparation, obturation and coronal seal. Apical one-third of all teeth samples were again immersed in the broth for 8 weeks to mimic secondary infection. The teeth were split into two halves and observed under confocal microscope. Results: E. faecalis penetrated 160 μm deep into the root cementum in group III samples and only showed adhesion in group II samples. Conclusion: Adhesion and Penetration of E. faecalis into the cementum could be the reason for persistent infection.

The article was published by
El artículo fue publicado por
Journal of Conservative Dentistry
Volume / Volumen: 17
Number / Número: 2
First and last pages / Páginas inicial-final: 119-123
Year / Año: 2014
Month / Mes: march

This journal, which is covered by SIIC Data Bases,
is part of the bibliographic collections of the Biblioteca Biomédica (BB) SIIC.
Esta revista, clasificada por SIIC Data Bases,
integra el acervo bibliográfico de la Biblioteca Biomédica (BB) SIIC.

Principal institution where the research took place
Institución principal de la investigación
AB Shetty Memorial Institute of Dental Sciences, Mangalore, Karnataka, India

Authors' Report

Crónica del Autor

Bibliographic references
Referencias bibliográficas

1. Molander A, Reit C, Dahlén G, Kvist T. Microbiological status of root-filled teeth with apical periodontitis. Int Endod J 31:1-7, 1998.
2. Hartke A, Giard JC, Laplace JM, Auffray Y. Survival of Enterococcus faecalis in an oligotrophic microcosm: Changes in morphology, development of general stress resistance, and analysis of protein synthesis. Appl Environ Microbiol 64:4238-45, 1998.
3. Evans M, Davies JK, Sundqvist G, Figdor D. Mechanisms involved in the resistance of Enterococcus faecalis to calcium hydroxide. Int Endod J 35:221-8, 2002.
4. Sedgley CM, Lennan SL, Appelbe OK. Survival of Enterococcus faecalis in root canals ex vivo. Int Endod J 38:735-42, 2005.
5. Chivatxaranukul P, Dashper SG, Messer HH. Dentinal tubule invasion and adherence by Enterococcus faecalis. Int Endod J 41:873-82, 2008.
6. Sen BH, Piskin B, Demirci T. Observation of bacteria and fungi in infected root canals and dentinal tubules by SEM. Endod Dent Traumatol 11:6-9, 1995.
7. Nallapareddy SR, Murray BE. Ligand-signaled upregulation of Enterococcus faecalis ace transcription, a mechanism for modulating host-E. faecalis interaction. Infect Immun 74:4982-9, 2006.
8. Hubble TS, Hatton JF, Nallapareddy SR, Murray BE, Gillespie MJ. Influence of Enterococcus faecalis proteases and the collagenbinding protein, Ace, on adhesion to dentin. Oral Microbiol Immunol 18:121-6, 2003.
9. Nallapareddy SR, Singh KV, Duh RW, Weinstock GM, Murray BE. Diversity of ace, a gene encoding a microbial surface component recognizing adhesive matrix molecules, from different strains of Enterococcus faecalis and evidence for production of ace during human infections. Infect Immun 68:5210-7, 2000.
10. Nallapareddy SR, Qin X, Weinstock GM, Höök M, Murray BE. Enterococcus faecalis adhesin, ace, mediates attachment to extracellular matrix proteins collagen type IV and laminin as well as collagen type I. Infect Immun 68:5218-24, 2000.
11. Cross SE, Kreth J, Wali RP, Sullivan R, Shi W, Gimzewski JK. Evaluation of bacteria-induced enamel demineralization using optical profilometry. Dent Mater 25:1517-26, 2009.
12. Zapata RO, Bramante CM, De Moraes IG, Bernardineli N, Gasparoto TH, Graeff MS, et al. Confocal laser scanning microscopy is appropriate to detect viability of Enterococcus faecalis in infected dentin. J Endod 34:1198-201, 2008.

Other articles written by the author Rahul Halkai
Otros artículos de Rahul Halkai

SIIC System of Assisted Editing (SSEA)
/ Sistema SIIC de Edición Asistida (SSEA)

Rahul Halkai was invited by SIIC on
Rahul Halkai fue invitado por SIIC el
2014, august 15
  The authors' report was accepted on
La crónica del autor fue aprobada el
2021, march 9
The authors' report was accepted on
La crónica del autor fue aprobada el
2021, march 9
Published in siicsalud
Publicado en siicsalud
2021, june 7

The article is strictly related to the following sections of siicsalud
El artículo se relaciona estrictamente con las siguientes secciones de siicsalud

Medical Education
/ Educación Médica
/ Odontología

and secondarily related to the following sections

y secundariamente con las siguientes secciones

Health Care Management
Administración Hospitalaria

Public Health
Salud Pública

Information about the full text
Acerca del trabajo completo

Evaluation of the Presence of Enterococcus Faecalis in Root Cementum: A Confocal Laser Scanning Microscope Analysis

Author / Autor
Rahul Halkai1, Mithra Hegde2, Kiran Halkai3

3 Dr., Hke’s Sn Dental College, Kalaburgi., Assistant Professor/ Sr. Lecture.

Access to the original source
Journal of Conservative Dentistry

Article URL:
/ URL del artículo:

URL of Abstract:
/ URL del abstract:

URL of Abstract page in Medline:
/ URL del abstract en Medline:

URL of Abstract page in Scielo:
/ URL del abstract en Scielo:

URL of Abstract page in Scopus:
/ URL del abstract en Scopus:

URL of Abstract page in Embase:
/ URL del abstract en Embase:

Other data bases:
/ Otras bases de datos:

siic DB:
/ siic DB: