DETECÇAO SIMULTANEA DOS GENES MECA E ILES-2 EM STAPHYLOCOCCUS COAGULASE NEGATIVOS ISOLADOS DE HOSPITAIS BRASILEIROS ATRAVES DA TECNICA DE PCR-MULTIPLEX





DETECÇAO SIMULTANEA DOS GENES MECA E ILES-2 EM STAPHYLOCOCCUS COAGULASE NEGATIVOS ISOLADOS DE HOSPITAIS BRASILEIROS ATRAVES DA TECNICA DE PCR-MULTIPLEX

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O estudo mostrou que o PCR-multiplex é necessário para determinar taxas mais precisas de resistência a oxacilina e a mupirocina em SCN, podendo ajudar no controle e prevenção de infecções estafilocócicas no Brasil.
regina9.jpg Autor:
Kátia Regina Netto Dos Santos,
Columnista Experto de SIIC

Institución:
Instituto de Microbiología Universidade Federal do Rio de Janeiro Rio de Janeiro, Brasil


Artículos publicados por Kátia Regina Netto Dos Santos,
Coautores
Rosana Barreto Rocha Ferreira*  Ana Paula Ferreira Nunes.*  Valeria Miguelote Kokis.*  Natascha Krepsky.*  Leila Souza Fonseca.**  Marcia Giambiagi de Marval.*** 
Mestre em MicrobiologiaInstituto de Microbiologia da Universidade Federal do Rio de Janeiro*
Professor Adjunto; Doutora em MicrobiologiaInstituto de Microbiologia da Universidade Federal do Rio de Janeiro**
Professora adjunto; Doutora em Microbiologia Instituto de Microbiologia da Universidade Federal do Rio de Janeiro***
Recepción del artículo
12 de Abril, 2004
Aprobación
8 de Junio, 2004
Primera edición
4 de Agosto, 2004
Segunda edición, ampliada y corregida
7 de Junio, 2021

Resumen
A susceptibilidade a oxacilina (Oxa) e a mupirocina (Mup) foi analisada por diferentes métodos em amostras hospitalares de SCN. Entre 112 amostras, 69 (61.6%) foram OxaR pelo teste de difusão do disco (DD) e 72 (64.2%) cresceram no agar triagem com 6 μg/ml de oxacilina. S. epidermidis e S. haemolyticus apresentaram taxas elevadas de resistência a oxacilina, 75.4% e 96.1%, respectivamente. Vinte e quatro (21.4%) amostras foram MupR pelo DD. A detecção dos genes mecA e ileS-2 por PCR-multiplex mostrou que 72 (64.2%) dos SCN possuíam o gene mecA e 16 (14.3%) apresentavam o gene ileS-2. Quinze dessas amostras apresentaram os dois genes simultaneamente. As amostras contendo o gene ileS-2 apresentavam CMI > 1 024 μg/mL pelo teste-E, enquanto resistência a baixos níveis de mupirocina (CMIs de 12–16 μg/mL) foi observada entre as amostras sem o ileS-2. A resistência a altos e baixos níveis de mupirocina não pode ser diferenciada pelo DD. A análise das amostras de S. epidermidis MupR por PFGE mostrou um perfil genotípico prevalente (80.9%) nos hospitais do Rio de Janeiro. O estudo mostrou que o PCR-multiplex é necessário para determinar taxas mais precisas de resistência a Oxa e a Mup em SCN, podendo ajudar no controle e prevenção de infecções estafilocócicas no Brasil.

Palabras clave
Staphylococcus coagulase negativos, resistência a mupirocina, resistência a oxacilina, PCR-multiplex, Eletroforese em gel de campo pulsado (PFGE)


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Abstract
Susceptibility to oxacillin and mupirocin was analyzed by different testing methods in clinical CNS isolates. Among 112 CNS strains, 69 (61.6%) were OxaR by the disk diffusion test (DD) and 72 (64.2%) grew on the oxacillin agar screen plate. S. epidermidis and S. haemolyticus presented high rates of oxacillin resistance (75.4 % and 96.1 %, respectively). Twenty four (21.4%) strains were MupR by the DD. The detection of the mecA and ileS-2 genes, determined by multiplex-PCR, showed that 72 (64.2%) CNS strains possessed the mecA gene and 16 (14.3%) possessed the ileS-2. Fifteen of these strains presented the two resistance genes simultaneously. The isolates containing the ileS-2 presented a MIC > 1 024 μg/mL in the E-test, while low-level mupirocin resistance (MICs of 12–16 μg/mL) was observed in strains without ileS-2. The resistances to high and low levels of mupirocin could not be distinguished by DD. The analysis of the MupR S. epidermidis strains by PFGE showed a prevalent profile (80.9%) in hospitals in Rio de Janeiro. This report showed that the multiplex-PCR is necessary to determine accurate rates of resistance to oxacillin and mupirocin in CNS, and will can help in the staphylococcal infections prevention and control policies in Brazil.

Key words
Coagulase-negative staphylococci; mupirocin r


Clasificación en siicsalud
Artículos originales > Expertos de Iberoamérica >
página   www.siicsalud.com/des/expertocompleto.php/

Especialidades
Principal: Diagnóstico por Laboratorio, Infectología
Relacionadas: Epidemiología, Medicina Interna, Salud Pública



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Bibliografía del artículo
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